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shrna cassettes  (Merck & Co)

 
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    Merck & Co shrna cassettes
    Shrna Cassettes, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shrna+cassettes/bio_rxiv__2025__09__15__676326-195-1-6?v=Merck+%26+Co
    Average 86 stars, based on 1 article reviews
    shrna cassettes - by Bioz Stars, 2026-08
    86/100 stars

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    Bioinformatic analysis of <t>LAIR1</t> expression in pan-cancer and its prognostic value in GC. (A) LAIR1 expression across cancers from TIMER2. (B-D) Poor prognosis associated with high LAIR1 expression (probe 208071_s_at) in GC: (B) OS, (C) FPS, (D) PPS. (E-G) Poor prognosis associated with high LAIR1 expression (probe 210644_s_at) in GC: (E) OS, (F) FPS, (G) PPS. *, P<0.05; ***, P<0.001. CI, confidence interval; FPS, first progression survival; GC, gastric cancer; HR, hazard ratio; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; OS, overall survival; PPS, post-progression survival; TPM, transcripts per million.
    Lair1 Overexpression Cassette, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    29 mer scrambled shrna cassette  (OriGene)
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    Bioinformatic analysis of <t>LAIR1</t> expression in pan-cancer and its prognostic value in GC. (A) LAIR1 expression across cancers from TIMER2. (B-D) Poor prognosis associated with high LAIR1 expression (probe 208071_s_at) in GC: (B) OS, (C) FPS, (D) PPS. (E-G) Poor prognosis associated with high LAIR1 expression (probe 210644_s_at) in GC: (E) OS, (F) FPS, (G) PPS. *, P<0.05; ***, P<0.001. CI, confidence interval; FPS, first progression survival; GC, gastric cancer; HR, hazard ratio; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; OS, overall survival; PPS, post-progression survival; TPM, transcripts per million.
    29 Mer Scrambled Shrna Cassette, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CD43 repression drives molecular events characteristic of germ cell differentiation. The human germ cell line TCam-2 was transfected with a linearized plasmid expressing <t>a</t> <t>non-effective</t> <t>shRNA</t> cassette (OriGene Technologies, Inc.). Stable expression of this plasmid was selected by purocmycin to generate the cell line TCam-43(+). TCam-2 was also transfected with a mixture of four plasmids expressing shRNA targeting human CD43 mRNA at 4 separate coding-strand sequences (OriGene Technologies, Inc.). Stable expression of these plasmids was selected by puromycin to generate TCam-43(-). Upper Panels: Total protein extracts were prepared from TCam-43(+) and TCam-43(-) and subjected to western blotting using the anti-CD43 antibody SSGZ , the anti-TNP1 antibody LS-C81760 (LifeSpan BioSciences, Inc., Seattle, WA) and the anti-Acrosin antibody ab125362 (Abcam, Inc., Cambridge, MA). The two proteins detected with anti-Acrosin are consistent with Preproacrosin (PP.Acrosin) and Proacrosin (P.Acrosin). Lower Panels: Cytoplasmic and nuclear protein extracts were prepared separately and analyzed with anti-Acrosin as above. Antibodies to Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and TATA-binding protein (TBP) were used to control for gel loading. Uncropped western blot images are presented in Supplementary file 1
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    shrna cassettes  (Merck & Co)
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    CD43 repression drives molecular events characteristic of germ cell differentiation. The human germ cell line TCam-2 was transfected with a linearized plasmid expressing <t>a</t> <t>non-effective</t> <t>shRNA</t> cassette (OriGene Technologies, Inc.). Stable expression of this plasmid was selected by purocmycin to generate the cell line TCam-43(+). TCam-2 was also transfected with a mixture of four plasmids expressing shRNA targeting human CD43 mRNA at 4 separate coding-strand sequences (OriGene Technologies, Inc.). Stable expression of these plasmids was selected by puromycin to generate TCam-43(-). Upper Panels: Total protein extracts were prepared from TCam-43(+) and TCam-43(-) and subjected to western blotting using the anti-CD43 antibody SSGZ , the anti-TNP1 antibody LS-C81760 (LifeSpan BioSciences, Inc., Seattle, WA) and the anti-Acrosin antibody ab125362 (Abcam, Inc., Cambridge, MA). The two proteins detected with anti-Acrosin are consistent with Preproacrosin (PP.Acrosin) and Proacrosin (P.Acrosin). Lower Panels: Cytoplasmic and nuclear protein extracts were prepared separately and analyzed with anti-Acrosin as above. Antibodies to Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and TATA-binding protein (TBP) were used to control for gel loading. Uncropped western blot images are presented in Supplementary file 1
    Shrna Cassettes, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    U6 Promoter Shrna Cassettes, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CD43 repression drives molecular events characteristic of germ cell differentiation. The human germ cell line TCam-2 was transfected with a linearized plasmid expressing <t>a</t> <t>non-effective</t> <t>shRNA</t> cassette (OriGene Technologies, Inc.). Stable expression of this plasmid was selected by purocmycin to generate the cell line TCam-43(+). TCam-2 was also transfected with a mixture of four plasmids expressing shRNA targeting human CD43 mRNA at 4 separate coding-strand sequences (OriGene Technologies, Inc.). Stable expression of these plasmids was selected by puromycin to generate TCam-43(-). Upper Panels: Total protein extracts were prepared from TCam-43(+) and TCam-43(-) and subjected to western blotting using the anti-CD43 antibody SSGZ , the anti-TNP1 antibody LS-C81760 (LifeSpan BioSciences, Inc., Seattle, WA) and the anti-Acrosin antibody ab125362 (Abcam, Inc., Cambridge, MA). The two proteins detected with anti-Acrosin are consistent with Preproacrosin (PP.Acrosin) and Proacrosin (P.Acrosin). Lower Panels: Cytoplasmic and nuclear protein extracts were prepared separately and analyzed with anti-Acrosin as above. Antibodies to Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and TATA-binding protein (TBP) were used to control for gel loading. Uncropped western blot images are presented in Supplementary file 1
    Gene Blocks Of The Dual Shrna Cassettes Containing Either Shscramble1 Shscramble2, Shscramble1 Shtxnip, Or Shrbms3 1 Shtxnip, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    gene blocks of the dual shrna cassettes containing either shscramble1-shscramble2, shscramble1-shtxnip, or shrbms3-1-shtxnip - by Bioz Stars, 2026-08
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    gfp shrna cassette  (Addgene inc)
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    Addgene inc gfp shrna cassette
    CD43 repression drives molecular events characteristic of germ cell differentiation. The human germ cell line TCam-2 was transfected with a linearized plasmid expressing <t>a</t> <t>non-effective</t> <t>shRNA</t> cassette (OriGene Technologies, Inc.). Stable expression of this plasmid was selected by purocmycin to generate the cell line TCam-43(+). TCam-2 was also transfected with a mixture of four plasmids expressing shRNA targeting human CD43 mRNA at 4 separate coding-strand sequences (OriGene Technologies, Inc.). Stable expression of these plasmids was selected by puromycin to generate TCam-43(-). Upper Panels: Total protein extracts were prepared from TCam-43(+) and TCam-43(-) and subjected to western blotting using the anti-CD43 antibody SSGZ , the anti-TNP1 antibody LS-C81760 (LifeSpan BioSciences, Inc., Seattle, WA) and the anti-Acrosin antibody ab125362 (Abcam, Inc., Cambridge, MA). The two proteins detected with anti-Acrosin are consistent with Preproacrosin (PP.Acrosin) and Proacrosin (P.Acrosin). Lower Panels: Cytoplasmic and nuclear protein extracts were prepared separately and analyzed with anti-Acrosin as above. Antibodies to Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and TATA-binding protein (TBP) were used to control for gel loading. Uncropped western blot images are presented in Supplementary file 1
    Gfp Shrna Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bioinformatic analysis of LAIR1 expression in pan-cancer and its prognostic value in GC. (A) LAIR1 expression across cancers from TIMER2. (B-D) Poor prognosis associated with high LAIR1 expression (probe 208071_s_at) in GC: (B) OS, (C) FPS, (D) PPS. (E-G) Poor prognosis associated with high LAIR1 expression (probe 210644_s_at) in GC: (E) OS, (F) FPS, (G) PPS. *, P<0.05; ***, P<0.001. CI, confidence interval; FPS, first progression survival; GC, gastric cancer; HR, hazard ratio; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; OS, overall survival; PPS, post-progression survival; TPM, transcripts per million.

    Journal: Translational Cancer Research

    Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

    doi: 10.21037/tcr-2026-1-0136

    Figure Lengend Snippet: Bioinformatic analysis of LAIR1 expression in pan-cancer and its prognostic value in GC. (A) LAIR1 expression across cancers from TIMER2. (B-D) Poor prognosis associated with high LAIR1 expression (probe 208071_s_at) in GC: (B) OS, (C) FPS, (D) PPS. (E-G) Poor prognosis associated with high LAIR1 expression (probe 210644_s_at) in GC: (E) OS, (F) FPS, (G) PPS. *, P<0.05; ***, P<0.001. CI, confidence interval; FPS, first progression survival; GC, gastric cancer; HR, hazard ratio; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; OS, overall survival; PPS, post-progression survival; TPM, transcripts per million.

    Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

    Techniques: Expressing

    Expression and prognostic analysis of LAIR1 in GC. (A) Representative images showing low LAIR1 expression in normal gastric tissue and high expression in GC tissue. Negative staining (IHC scores 1–2), weak staining (IHC scores 3–4), moderate staining (IHC scores 6–9), and strong staining (IHC scores 12). (B) OS based on LAIR1 expression status. (C) PFS based on LAIR1 expression status. (D) LAIR1 protein expression in 16 paired GC tissues (N: adjacent normal; T: tumor). (E) LAIR1 mRNA levels in the same 16 paired tissues. ****, P<0.0001. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GC, gastric cancer; IHC, immunohistochemistry; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; mRNA, messenger RNA; OS, overall survival; PFS, progression-free survival.

    Journal: Translational Cancer Research

    Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

    doi: 10.21037/tcr-2026-1-0136

    Figure Lengend Snippet: Expression and prognostic analysis of LAIR1 in GC. (A) Representative images showing low LAIR1 expression in normal gastric tissue and high expression in GC tissue. Negative staining (IHC scores 1–2), weak staining (IHC scores 3–4), moderate staining (IHC scores 6–9), and strong staining (IHC scores 12). (B) OS based on LAIR1 expression status. (C) PFS based on LAIR1 expression status. (D) LAIR1 protein expression in 16 paired GC tissues (N: adjacent normal; T: tumor). (E) LAIR1 mRNA levels in the same 16 paired tissues. ****, P<0.0001. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GC, gastric cancer; IHC, immunohistochemistry; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; mRNA, messenger RNA; OS, overall survival; PFS, progression-free survival.

    Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

    Techniques: Expressing, Negative Staining, Staining, Immunohistochemistry

    LAIR1 expression and its impact on GC cell proliferation. (A) LAIR1 protein levels in various GC cell lines. (B) Statistical analysis of LAIR1 expression across cell lines. (C) Validation of LAIR1 knockdown efficiency via lentivirus in MGC803 cells. (D) Validation of LAIR1 overexpression efficiency in MKN45 cells. (E) Effect of LAIR1 knockdown on cell viability (CCK-8). (F) Effect of LAIR1 overexpression on cell viability (CCK-8). (G) Colony formation after LAIR1 knockdown in MGC803 cells (crystal violet staining). (H) Colony formation after LAIR1 overexpression in MKN45 cells (crystal violet staining). **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant. CCK-8, Cell Counting Kit-8; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GC, gastric cancer; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; sh-1, shLAIR1-1; sh-2, shLAIR1-2; shLAIR1, short hairpin RNA targeting LAIR1.

    Journal: Translational Cancer Research

    Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

    doi: 10.21037/tcr-2026-1-0136

    Figure Lengend Snippet: LAIR1 expression and its impact on GC cell proliferation. (A) LAIR1 protein levels in various GC cell lines. (B) Statistical analysis of LAIR1 expression across cell lines. (C) Validation of LAIR1 knockdown efficiency via lentivirus in MGC803 cells. (D) Validation of LAIR1 overexpression efficiency in MKN45 cells. (E) Effect of LAIR1 knockdown on cell viability (CCK-8). (F) Effect of LAIR1 overexpression on cell viability (CCK-8). (G) Colony formation after LAIR1 knockdown in MGC803 cells (crystal violet staining). (H) Colony formation after LAIR1 overexpression in MKN45 cells (crystal violet staining). **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant. CCK-8, Cell Counting Kit-8; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GC, gastric cancer; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; sh-1, shLAIR1-1; sh-2, shLAIR1-2; shLAIR1, short hairpin RNA targeting LAIR1.

    Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

    Techniques: Expressing, Biomarker Discovery, Knockdown, Over Expression, CCK-8 Assay, Staining, Cell Counting, Negative Control, shRNA

    Effect of LAIR1 on GC cell migration and invasion. (A) Scratch wound assay in LAIR1-knockdown MGC803 cells. (B) Scratch wound assay in LAIR1-overexpressing MKN45 cells. (C) Transwell invasion assay in LAIR1-knockdown MGC803 cells (crystal violet staining). (D) Transwell invasion assay in LAIR1-overexpressing MKN45 cells (crystal violet staining). ***, P<0.001; ****, P<0.0001. GC, gastric cancer; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; sh-1, shLAIR1-1; sh-2, shLAIR1-2; shLAIR1, short hairpin RNA targeting LAIR1.

    Journal: Translational Cancer Research

    Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

    doi: 10.21037/tcr-2026-1-0136

    Figure Lengend Snippet: Effect of LAIR1 on GC cell migration and invasion. (A) Scratch wound assay in LAIR1-knockdown MGC803 cells. (B) Scratch wound assay in LAIR1-overexpressing MKN45 cells. (C) Transwell invasion assay in LAIR1-knockdown MGC803 cells (crystal violet staining). (D) Transwell invasion assay in LAIR1-overexpressing MKN45 cells (crystal violet staining). ***, P<0.001; ****, P<0.0001. GC, gastric cancer; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; sh-1, shLAIR1-1; sh-2, shLAIR1-2; shLAIR1, short hairpin RNA targeting LAIR1.

    Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

    Techniques: Migration, Scratch Wound Assay Assay, Knockdown, Transwell Invasion Assay, Staining, Negative Control, shRNA

    Effect of LAIR1 knockdown on subcutaneous tumor growth in nude mice. (A) Tumorigenesis in nude mice following LAIR1 downregulation. (B,C) Tumor progression: significant differences in subcutaneous tumor growth curves (B) and final tumor weights (C). (D) IHC scores for LAIR1 and Ki-67. (E) Representative IHC images for LAIR1. (F) Representative IHC images for Ki-67. **, P<0.01; ****, P<0.0001. IHC, immunohistochemistry; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; shLAIR1, short hairpin RNA targeting LAIR1.

    Journal: Translational Cancer Research

    Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

    doi: 10.21037/tcr-2026-1-0136

    Figure Lengend Snippet: Effect of LAIR1 knockdown on subcutaneous tumor growth in nude mice. (A) Tumorigenesis in nude mice following LAIR1 downregulation. (B,C) Tumor progression: significant differences in subcutaneous tumor growth curves (B) and final tumor weights (C). (D) IHC scores for LAIR1 and Ki-67. (E) Representative IHC images for LAIR1. (F) Representative IHC images for Ki-67. **, P<0.01; ****, P<0.0001. IHC, immunohistochemistry; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; shLAIR1, short hairpin RNA targeting LAIR1.

    Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

    Techniques: Knockdown, Immunohistochemistry, Negative Control, shRNA

    Effect of LAIR1 knockdown on peritoneal metastasis in nude mice. (A) Representative images of peritoneal metastatic nodules. (B) The number of nodules in the peritoneal metastases of nude mice exhibited significant variations. (C) IHC scores for LAIR1, Snail1, N-cadherin, and E-cadherin. (D-G) Representative IHC images for LAIR1 (D), Snail1 (E), N-cadherin (F), and E-cadherin (G). *, P<0.05; **, P<0.01; ****, P<0.0001. IHC, immunohistochemistry; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; shLAIR1, short hairpin RNA targeting LAIR1.

    Journal: Translational Cancer Research

    Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

    doi: 10.21037/tcr-2026-1-0136

    Figure Lengend Snippet: Effect of LAIR1 knockdown on peritoneal metastasis in nude mice. (A) Representative images of peritoneal metastatic nodules. (B) The number of nodules in the peritoneal metastases of nude mice exhibited significant variations. (C) IHC scores for LAIR1, Snail1, N-cadherin, and E-cadherin. (D-G) Representative IHC images for LAIR1 (D), Snail1 (E), N-cadherin (F), and E-cadherin (G). *, P<0.05; **, P<0.01; ****, P<0.0001. IHC, immunohistochemistry; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; shLAIR1, short hairpin RNA targeting LAIR1.

    Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

    Techniques: Knockdown, Immunohistochemistry, Negative Control, shRNA

    Effect of LAIR1 knockdown on lung and lymph node metastasis in nude mice. (A) Gross view of lung metastasis. (B) Lung metastatic nodules visualized by H&E staining. (C) Quantitative analysis of metastatic lung nodules. (D) Gross view of lymph node metastasis. (E) Quantitative analysis of metastatic lymph nodes. **, P<0.01; ****, P<0.0001. H&E, hematoxylin-eosin; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; shLAIR1, short hairpin RNA targeting LAIR1.

    Journal: Translational Cancer Research

    Article Title: Unveiling LAIR1: a prognostic biomarker associated with gastric cancer progression and metastasis

    doi: 10.21037/tcr-2026-1-0136

    Figure Lengend Snippet: Effect of LAIR1 knockdown on lung and lymph node metastasis in nude mice. (A) Gross view of lung metastasis. (B) Lung metastatic nodules visualized by H&E staining. (C) Quantitative analysis of metastatic lung nodules. (D) Gross view of lymph node metastasis. (E) Quantitative analysis of metastatic lymph nodes. **, P<0.01; ****, P<0.0001. H&E, hematoxylin-eosin; LAIR1, leukocyte-associated immunoglobulin-like receptor 1; NC, negative control; shLAIR1, short hairpin RNA targeting LAIR1.

    Article Snippet: To modulate LAIR1 expression, lentiviral vectors carrying short hairpin RNA targeting LAIR1 (shLAIR1), a nontargeting scrambled negative control short hairpin RNA (shNC), or a LAIR1 overexpression cassette were constructed and supplied by Shanghai GeneChem Co., Ltd. (Shanghai, China).

    Techniques: Knockdown, Staining, Negative Control, shRNA

    CD43 repression drives molecular events characteristic of germ cell differentiation. The human germ cell line TCam-2 was transfected with a linearized plasmid expressing a non-effective shRNA cassette (OriGene Technologies, Inc.). Stable expression of this plasmid was selected by purocmycin to generate the cell line TCam-43(+). TCam-2 was also transfected with a mixture of four plasmids expressing shRNA targeting human CD43 mRNA at 4 separate coding-strand sequences (OriGene Technologies, Inc.). Stable expression of these plasmids was selected by puromycin to generate TCam-43(-). Upper Panels: Total protein extracts were prepared from TCam-43(+) and TCam-43(-) and subjected to western blotting using the anti-CD43 antibody SSGZ , the anti-TNP1 antibody LS-C81760 (LifeSpan BioSciences, Inc., Seattle, WA) and the anti-Acrosin antibody ab125362 (Abcam, Inc., Cambridge, MA). The two proteins detected with anti-Acrosin are consistent with Preproacrosin (PP.Acrosin) and Proacrosin (P.Acrosin). Lower Panels: Cytoplasmic and nuclear protein extracts were prepared separately and analyzed with anti-Acrosin as above. Antibodies to Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and TATA-binding protein (TBP) were used to control for gel loading. Uncropped western blot images are presented in Supplementary file 1

    Journal: Reproductive Biology and Endocrinology : RB&E

    Article Title: CD43 expressed in Sertoli, Leydig and germ cells inhibits testicular function and represents a potential target to treat male infertility or effect male contraception

    doi: 10.1186/s12958-025-01410-2

    Figure Lengend Snippet: CD43 repression drives molecular events characteristic of germ cell differentiation. The human germ cell line TCam-2 was transfected with a linearized plasmid expressing a non-effective shRNA cassette (OriGene Technologies, Inc.). Stable expression of this plasmid was selected by purocmycin to generate the cell line TCam-43(+). TCam-2 was also transfected with a mixture of four plasmids expressing shRNA targeting human CD43 mRNA at 4 separate coding-strand sequences (OriGene Technologies, Inc.). Stable expression of these plasmids was selected by puromycin to generate TCam-43(-). Upper Panels: Total protein extracts were prepared from TCam-43(+) and TCam-43(-) and subjected to western blotting using the anti-CD43 antibody SSGZ , the anti-TNP1 antibody LS-C81760 (LifeSpan BioSciences, Inc., Seattle, WA) and the anti-Acrosin antibody ab125362 (Abcam, Inc., Cambridge, MA). The two proteins detected with anti-Acrosin are consistent with Preproacrosin (PP.Acrosin) and Proacrosin (P.Acrosin). Lower Panels: Cytoplasmic and nuclear protein extracts were prepared separately and analyzed with anti-Acrosin as above. Antibodies to Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and TATA-binding protein (TBP) were used to control for gel loading. Uncropped western blot images are presented in Supplementary file 1

    Article Snippet: The human germ cell line TCam-2 was transfected with a linearized plasmid expressing a non-effective shRNA cassette (OriGene Technologies, Inc.).

    Techniques: Cell Differentiation, Transfection, Plasmid Preparation, Expressing, shRNA, Western Blot, Binding Assay, Control